Based on the reads obtained from a 454 run we have identified numerous microsatellites in Funaria hygrometrica and Entosthodon obtusus. These primer sequences are publicly available. We would appreciate if you acknowledge this site as the source of the data:
Liu Y. & B. Goffinet. 2012. Microsatellite sites identified from 454 reads from entire genomic extracts in Funaria hygrometrica and Entosthodon obtusus.
TABLE 1. Primer information of the newly targeted mitochondrial markers. All the primers listed were designed for the current study.
Region |
Productsize a (bp) |
T a (˚C) |
Primername b |
Primer sequences(5 ′ -3 ′ ) |
| atp1-trnW | 759 | 48 | atp1-F | TTTCTGCTATCGTACAAC |
| trnW-R | AATGGTAGAACAATGGTC | |||
| 654 | 50 | atp1-F1* | AGTCAACTGGCTACC | |
| trnW-R1* | ACAGGTTAAGGGTTC | |||
| atp9-trnI | 628 | 48 | atp9-F | CTATCCAACCTCATTCTG |
| trnI-R | CGAACCTACAATATCACC | |||
| 523 | 50 | atp9-F1* | TGTTTGCCTTAATGATGG | |
| trnI-R1* | TTATGAGCGGTGCGTTTG | |||
| nad1i287 | 1225 | 50 | nad1-F | GTGGGATTGTTTGGATTG |
| nad1-R | GTGGGATTGTTTGGATTG | |||
| 742 | 52 | nad1-F1* | TGCTTGGGCTGTTATACC | |
| nad1-R1* | GTAGGGGTCGGAGATTTC | |||
| nad2-trnG | 703 | 55 | nad2-F | AGGCTGTGGGGCYTACTTAC |
| trnG-R | AAGGCTRAGGTTGAGGGTTC | |||
| rpl2 | 1184 | 52 | rpl2-F | TTCATCAGGGCGTATTAC |
| rpl2-R | CGGATTCATAGCAACACC | |||
| 813 | 52 | rpl2-F1* | AACACTTCGTCTATTGGC | |
| rpl2-R1* | GATACGCAATTTCCTACC | |||
| rpl5-16 | 1266 | 52 | rpl16-F | CCAGTAAACCCACCGAAG |
| rpl5-R | AGAGTGCTTTGTGCTAGG | |||
| 890 | 54 | rpl16-F1* | GGATGGTGTGAGTTTGTC | |
| rpl5-R1* | CGGAGTCTATTTGGAGTG | |||
| rps3 | 1503 | 48 | rps3-F | TCGTAGTTCAGATTCAAG |
| rps3–R | CACCTAAAATCCCATAAG | |||
| 1040 | 50 | rps3-F1* | GCAATACAATCGGTCAAG | |
| rps3–R1* | GAGCAATTAGAGAAGCAC | |||
| rps7-atp6 | 1178 | 50 | rps7-F | AAGCCTRTTTGTGAAGTGAA |
| atp6-R | CTGGTCATAGTTTAGTMAAG | |||
| 1068 | 52 | rps7-F1* | TATRGCARCAAATCGTCAAG | |
| atp6-R1* | TATCTATGGGGGGTATTATG |